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M9490553.TXT
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1994-09-24
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Document 0553
DOCN M9490553
TI Reassessment of the differential effects of ultraviolet and ionizing
radiation on HIV promoter: the use of cell survival as the basis for
comparisons.
DT 9411
AU Beer JZ; Olvey KM; Lee W; Zmudzka BZ; Center for Devices and
Radiological Health, Food and Drug; Administration, Rockville, MD 20857.
SO Photochem Photobiol. 1994 Jun;59(6):643-9. Unique Identifier : AIDSLINE
MED/94344998
AB Effects of different radiation treatments on the human immunodeficiency
virus-1 (HIV) promoter were reassessed for exposures comparable to those
encountered in clinical or cosmetic practice, using survival of the host
cell as a basis for comparisons. The exposures were performed with two
ultraviolet radiation sources commonly used as medical or cosmetic
devices (UVASUN 2000 and FS20 lamps), a germicidal (G15T8) lamp and an
X-ray machine. The UVC component of the FS20 lamp was filtered out. The
emission spectra of the lamps were determined. The characteristics of
these sources allowed us to discriminate among effects of UVA1 (340-400
nm), UVB + UVA2 (280-340 nm) and UVC (254 nm) radiations. Effects of
irradiation were ascertained using cultures of HeLa cells stably
transfected with the HIV promoter linked to a reporter-chloramphenicol
acetyl transferase-gene. The exposures used caused at least two logs of
cell killing. In this cytotoxicity range, UVA1 or X radiations had no
effect on the HIV promoter, whereas UVB + UVA2 or UVC radiations
activated the HIV promoter in a fluence-dependent manner. Survivals
following exposure to UVB + UVA2 or UVC radiation were (1) at the lowest
measurable HIV promoter activation, 30 and 20%, respectively, (2) at
one-half maximal activation, 6 and 3%, respectively and (3) at the
maximal activation, 0.5 and 0.2%, respectively.(ABSTRACT TRUNCATED AT
250 WORDS)
DE Cell Survival/RADIATION EFFECTS Comparative Study Gene Expression
Regulation, Viral/RADIATION EFFECTS Hela Cells Human
HIV-1/GENETICS/*RADIATION EFFECTS Promoter Regions
(Genetics)/*RADIATION EFFECTS Support, U.S. Gov't, Non-P.H.S. Support,
U.S. Gov't, P.H.S. Ultraviolet Rays JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).